Microscope Resolution Oil Immersion – Rankers Physics
Topic: Ray Optics
Subtopic: Refraction by Spherical Surfaces

Microscope Resolution Oil Immersion

Assertion (A): For better resolution, in microscope we prefer "Oil immersed object".
Reason (R): Refractive index of oil is closer to the refractive index of objective glass.
 
(1) Both (A) & (R) are true and the (R) is the correct explanation of the (A)
(2) Both (A) & (R) are true but the (R) is not the correct explanation of the (A)
(3) (A) is true but (R) is false
(4) Both (A) and (R) are false

Solution:

Oil immersion is used in microscopes to increase the numerical aperture (NA) and thus improve resolution. Thus, (A) is true.
The refractive index of the immersion oil is chosen to be very close to that of the objective lens glass, which minimizes refraction and maximizes the angle of light collected, increasing NA. Thus, (R) is true and is the correct explanation of (A).

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